LOCUS       Exported                4460 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0096_pluxRRR12y-sfGFP-maxRBS-luxI
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="R2oDNA spacer"
                     /note="R2oDNA 70bp spacer 13"
     terminator      203..230
                     /note="T7Te"
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(238..308)
                     /note="taR12y"
     promoter        complement(309..363)
                     /note="lux rR (R0062)"
     misc_feature    364..433
                     /note="spacer (B0040)"
     promoter        434..488
                     /note="lux rR (R0062)"
     3'UTR           489..540
                     /note="crR12y"
     RBS             523..534
                     /note="RBS (B0034)"
     CDS             541..1266
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     RBS             1287..1291
                     /note="RBS (B0034)"
     CDS             1299..1883
                     /codon_start=1
                     /note="luxI (C0161)"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*"
     terminator      1895..1981
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1975..2002
                     /note="T1"
     primer_bind     2009..2028
                     /note="AB179"
     primer_bind     2194..2213
                     /note="AB5"
     rep_origin      2347..3174
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      2595..2597
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(2598..3142)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     3246..3265
                     /note="AB180"
     oriT            3276..3384
                     /note="incP origin of transfer"
     misc_feature    3356
                     /note="C->T no real mutation, just for testing"
     CDS             complement(3540..4355)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg
      181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct
      241 agagatatat ggtagtagta agttaatttt cattaaccac cactaccaat cacctcctgg
      301 atttagcttt tattcgacta taacaaacca ttttcttgcg taaacctgta cgatcctaca
      361 ggtaggttct gttaagtaac tgaacccaat gtcgttagtg acgcttacct cttaagaggt
      421 cactgaccta acaacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt
      481 cgaataaaga attctaccat tcacctcttg gatttagcta ttaaagagga gaaaggtacc
      541 atgagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      601 gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga
      661 aaactcaccc ttaaatttat ttgcactact ggaaaactac ctgttccgtg gccaacactt
      721 gtcactactc tgacctatgg tgttcaatgc ttttcccgtt atccggatca catgaaacgg
      781 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      841 aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      901 aatcgtatcg agttaaaggg tattgatttt aaagaagatg gaaacattct tggacacaaa
      961 ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     1021 atcaaagcta acttcaaaat tcgccacaac gttgaagatg gttccgttca actagcagac
     1081 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1141 ctgtcgacac aatctgtcct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     1201 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaaaggcct
     1261 taataaagcg aaacacgcat attttaagga ggtactacat gactataatg ataaaaaaat
     1321 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag
     1381 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt
     1441 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat
     1501 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc
     1561 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag
     1621 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag
     1681 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag
     1741 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa
     1801 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta
     1861 aaaaagcagt cttaaattaa taaattaaag gcatcaaata aaacgaaagg ctcagtcgaa
     1921 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa
     1981 tccgccgccc tagacagctg ggcgcgccaa atacgcccgg tagtgatctt atttcattat
     2041 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc
     2101 agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg
     2161 tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt
     2221 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
     2281 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
     2341 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
     2401 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
     2461 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     2521 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     2581 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     2641 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg
     2701 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     2761 tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc
     2821 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac
     2881 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt
     2941 aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac
     3001 aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca
     3061 gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac
     3121 gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagca
     3181 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3241 ttccccgaaa agtgccacct gctagggccg gcctacggcc agcctcgcag agcaggattc
     3301 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgtgggt
     3361 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     3421 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     3481 gctataatga ccccgaagca gggttatgca gcggaaaagg acccttgtcc aattaattat
     3541 tagaaaaatt catccagcat cagatgaaat tgcagtttgt tcatatccgg attatcaatg
     3601 ccatatttct gaaacagacg tttttgcagg ctcgggctaa attcgcccag gcagttccac
     3661 agaatggcca gatcctgata acgatccgca atgcccacac ggcccacatc aatgcagcca
     3721 atcagtttgc cttcatcgaa aatcaggtta tccaggctaa aatcgccgtg ggtcaccacg
     3781 ctatccgggc taaacggcag cagtttatgc atttctttcc acacctgttc caccggccag
     3841 ccgttacgtt catcatcaaa atcgctcgca tccaccaggc cgttgttcat acggctctgc
     3901 gcctgggcca gacgaaacac acgatcgctg ttaaacgggc agttgcacac cggaatgcta
     3961 tgcagacgac gcagaaacac ggccagcgca tccacaatgt tttcgccgct atccggatat
     4021 tcttccagca cctgaaacgc ggttttgccc ggaatcgcgg tggtcagcag ccacgcatca
     4081 tccggggtgc gaataaaatg tttaatggtc ggcagcggca taaattcggt cagccagttc
     4141 agacgcacca tttcatcggt cacatcgttc gccacgctgc ctttgccatg tttcagaaac
     4201 agttccggcg catccggttt gccatacaga cgataaatgg tcgcgccgct ctgacccacg
     4261 ttatcacgcg cccatttata gccatacaga tccgcatcca tgttgctgtt cagacgcgga
     4321 cggctacagc tcgtttcacg ctgaatatgg ctcataacac cccttgtatt actgtttatg
     4381 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     4441 agattttgag acacaaattt
//
